37515NWH9U

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Cells were seeded on 100-mm culture dishes and treated with doxorubicin, tetrac or both for 24 h, after which the cells were subjected to trypsin treatment at 37° C. for 3 min, fixed with 4% formalin in PBS, and washed with PBS. Then they were incubated with 0.1 μg/ml Hoechst 33248 (bisbenzimide, Sigma-Aldrich) and spotted on slides for microscopy. Positive cells are counted and their fractions between non treated and treated populations are compared. at 37° C. for 3 min washed with PBS their fractions between non treated treated populations

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Cells were seeded on 100-mm culture dishes and treated with doxorubicin, tetrac or both for 24 h, after which the cells were subjected to trypsin treatment at 37° C. for 3 min, fixed with 4% formalin in PBS, and washed with PBS. Then they were incubated with 0.1 μg/ml Hoechst 33248 (bisbenzimide, Sigma-Aldrich) and spotted on slides for microscopy. Positive cells are counted and their fractions between non treated and treated populations are compared. at 37° C. for 3 min washed with PBS their fractions between non treated treated populations

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: β-D-galactose (10 g, 1 eq), heptylamine (7.7 g, 1 eq) and water (2 g, 3 eq) were heated in a reaction flask at 80° C. for about 15 minutes. The sample was filtered and washed as described for the decyl derivative to give an 87% yield. The sample was filtered washed to give an 87% yield

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: β-D-galactose (10 g, 1 eq), decylamine (8.7 g, 1 eq), and water (2 g, 3eq) were heated in a reaction flask for 20 min at 80° C. Methanol/ethyl ether (50 v/50 v) was added, the product was filtered, washed with ethyl ether (2×50 ml) and dried, to give a 90% yield. the product was filtered washed with ethyl ether (2×50 ml) dried to give a 90% yield

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: β-D-galactose (10 g, 1 eq), decylamine (8.7 g, 1 eq), and water (2 g, 3eq) were heated in a reaction flask for 20 min at 80° C. Methanol/ethyl ether (50 v/50 v) was added, the product was filtered, washed with ethyl ether (2×50 ml) and dried, to give a 90% yield. the product was filtered washed with ethyl ether (2×50 ml) dried to give a 90% yield

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: To the Fab' (1 mg) prepared in the above process (a) was added the β-Gal-maleimide (3.9 mg) prepared in the process (b) and the mixture was reacted at 30° C. for 30 minutes. The reaction solution was centrifuged and the supernatant was subjected to Sepharose 6B column (φ1.5 cm×50 cm, eluent: 10 mM phosphate buffer, pH 6.5, containing 0.1% BSA) to give a β-Gal-Fab' fragment. the mixture was reacted at 30° C. for 30 minutes

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Synthesis of NeuAcα2-6Galβ1-4GlcNH2 βSEt. Galβ1-4GlcNH2 βSEt is prepared as described above and used directly or after isolation as acceptor for a β-D-galactoside α2-6-sialyltransferase (e.g. EC 2.4.99.1) reaction with a suitable glycosyl donor such as CMP-NeuAc.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: α-D-galactopyranose; β-D-galactopyranose; α-D-galactofuranose; β-D-galactofuranose; L-galactose; α-L-galactopyranose; β-L-galactopyranose; α-L-galactofuranose; β-L-galactofuranose

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Cells were seeded on 100-mm culture dishes and treated with doxorubicin, tetrac or both for 24 h, after which the cells were subjected to trypsin treatment at 37° C. for 3 min, fixed with 4% formalin in PBS, and washed with PBS. Then they were incubated with 0.1 μg/ml Hoechst 33248 (bisbenzimide, Sigma-Aldrich) and spotted on slides for microscopy. Positive cells are counted and their fractions between non treated and treated populations are compared. at 37° C. for 3 min washed with PBS their fractions between non treated treated populations