1,4-dichloro-2-(chloromethyl)benzene

Conditions: temp:0.00; pressure:0.00; stir:

Conditions: temp:0.00; pressure:0.00; stir:

Conditions: temp:0.00; pressure:0.00; stir:

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 32 Parts of 4-chlorobenzothietane-2-spiro-2'-imidazolidine is reacted as described in Example 11 with 29.5 parts of 2,5-dichlorobenzyl chloride in 300 parts of methanol. The yield is 53 parts (86% of theory) and the melting point is 260° C. with decomposition.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 32 Parts of 4-chlorobenzothietane-2-spiro-2'-imidazolidine is reacted as described in Example 11 with 29.5 parts of 2,5-dichlorobenzyl chloride in 300 parts of methanol. The yield is 53 parts (86% of theory) and the melting point is 260° C. with decomposition.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The title compound was prepared in the manner analogous to Example 1F using 12C and 1,4-Dichloro-2-(4-chloromethyl-phenoxymethyl)-benzene prepared from 1,4-dichloro-2-chloromethyl-benzene and 4-hydroxymethyl-phenol in the manner analagous to Examples 14A and 14B. MS m/z 265 (M−237).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The title compound was prepared in the manner analogous to Example 1F using 12C and 1,4-Dichloro-2-(4-chloromethyl-phenoxymethyl)-benzene prepared from 1,4-dichloro-2-chloromethyl-benzene and 4-hydroxymethyl-phenol in the manner analagous to Examples 14A and 14B. MS m/z 265 (M−237).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A mixture of ethyl 6-amino-9H-β-carboline-3-carboxylate (100 mg, 0.39 mmol), NaH (0.58 mmol, 24 mg of 60 wt. % suspension in mineral oil) and 2,5-dichlorobenzyl chloride (115 mg, 0.59 mmol) in DMF (2 mL), was treated according to Method A. Purification of the crude product by column chromatography on silica with hexane/ethyl acetate (7:3) as eluent furnished the desired product (87 mg, 54% yield) as a yellow solid. 1H NMR (DMSO-d6) δ8.96 (s, 1H), 8.74 (s,1H), 7.60 (d,J=8.6 Hz, 1H), 7.48 (d, 1H), 7.41 (dd, J=8.4 Hz, 2.1Hz, 1H), 7.35 (d, J=8.8 Hz, 1H), 7.01 (d,J=8.6 Hz, 1H), 6.51 (d, J=1.8 Hz, 1H), 5.80 (s, 2H), 5.10 (br s, 1H), 4.37 (q,J=7.0 Hz, 2H), 1.37 (t, J=7.0 Hz, 3H); MS (APCI; (M+H)+) m/z 414. was treated Purification of the crude product by column chromatography on silica with hexane/ethyl acetate (7:3) as eluent

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 6-(2,5-Dichlorobenzyl)-1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one was prepared using Method A from 1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one (9 mg, 0.038 mmol), NaH (0.046 mmol, 2 mg of 60 wt. % suspension in mineral oil) and 2,5-dichlorobenzyl chloride (9 mg, 0.046 mmol) in DMF (1 mL). Purification of the crude product by preparative thin layer chromatography with hexane/ether (2:1) as eluent furnished the desired product (5 mg, 33% yield) as a yellow solid. 1H NMR (CDCl3) δ8.93 (s, 1H), 8.09 (d, J=8.0 Hz, 1H), 7.65 (t, J=7.8 Hz, 1H), 7.37-7.46 (m, 3H), 7.16 (d,J=2.2 Hz, 1H), 6.42 (d,J=2.2 Hz, 1H), 6.05 (q,J=6.6 Hz, 1H), 5.70 (s, 2H), 1.92 (d,J=6.6 Hz, 3H); MS (APCI; (M+H)+) m/z 397. 6-(2,5-Dichlorobenzyl)-1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one was prepared Purification of the crude product by preparative thin layer chromatography with hexane/ether (2:1) as eluent

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 6-(2,5-Dichlorobenzyl)-1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one was prepared using Method A from 1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one (9 mg, 0.038 mmol), NaH (0.046 mmol, 2 mg of 60 wt. % suspension in mineral oil) and 2,5-dichlorobenzyl chloride (9 mg, 0.046 mmol) in DMF (1 mL). Purification of the crude product by preparative thin layer chromatography with hexane/ether (2:1) as eluent furnished the desired product (5 mg, 33% yield) as a yellow solid. 1H NMR (CDCl3) δ8.93 (s, 1H), 8.09 (d, J=8.0 Hz, 1H), 7.65 (t, J=7.8 Hz, 1H), 7.37-7.46 (m, 3H), 7.16 (d,J=2.2 Hz, 1H), 6.42 (d,J=2.2 Hz, 1H), 6.05 (q,J=6.6 Hz, 1H), 5.70 (s, 2H), 1.92 (d,J=6.6 Hz, 3H); MS (APCI; (M+H)+) m/z 397. 6-(2,5-Dichlorobenzyl)-1-methyl-1,6-dihydro-3H-furo[3′,4′:5,6]pyrido[3,4-b]indol-3-one was prepared Purification of the crude product by preparative thin layer chromatography with hexane/ether (2:1) as eluent

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: To a suspension of sodium hydride (1.60 g of 60% dispersion in oil, 0.04 mol) in dry DMF (60 mL) was added methyl 9H-β-carboline-3-carboxylate (8.7 g, 0.0385 mol) at −20° C. with stirring. The mixture was allowed to warm to room temperature with stirring under nitrogen. After stirring for 10 minutes at room temperature, formation of the sodium salt of the β-carboline appeared to be complete, resulting in a clear brown solution. The reaction mixture was cooled in an ice bath, 2,5-dichlorobenzyl chloride (7.82 g, 0.040 mol) was added, and the mixture was allowed to warm to room temperature and stirred overnight. The reaction mixture was diluted with water (200 mL), filtered, and the cake washed with water, ethyl acetate, ether, and dried, affording 13.05 g (88%) of the title product. 1H NMR (CDCl3) δ8.88 (s, 1H), 8.78 (s, 1H), 8.20 (d, J=7.8 Hz, 1H), 7.61 (t, J=7.8 Hz, 1H), 7.45-7.35 (m, 3H) 7.18 (dd, J=8.2, 1.7 Hz,1H), 6.52 (s, 1H), 5.56 (s, 2H), 4.06 (s, 3H); MS (APCI; (M+H)+) m/z 385. with stirring under nitrogen After stirring for 10 minutes at room temperature resulting in a clear brown solution The reaction mixture was cooled in an ice bath to warm to room temperature stirred overnight filtered the cake washed with water, ethyl acetate, ether dried

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A suspension of ethyl 9H-β-carboline-3-carboxylate (2.40 g, 10 mmol) in anhydrous DMF (20 mL) was cooled in an ice bath under nitrogen. Sodium hydride (420 mg of 60% NaH in mineral oil, 10.5 mmol) was added at once, and the mixture stirred until most of the solids dissolved and hydrogen evolution stopped (approx 10 min). The cool mixture was treated with 2,5-dichlorobenzyl chloride (2.15 g, 1.0 mmol) slowly with stirring, then allowed to warm to room temperature over two hours. The resulting cloudy solution was neutralized with acetic acid, then the solvent was removed in vacuo, affording a tan solid (3.94 g). The crude material was chromatographed on silica gel using a mixture of chloroform and ethyl acetate, affording the title product (1.96 g, 49%). 1H NMR (300 MHz, DMSO-d6) δ9.06 (s, 1 H), 8.96 (s, 1H), 8.48 (d, J=8.0, 1H), 7.55-7.64 (m, 3H), 7.35-7.42 (m, 2H), 6.60 (d,J=2.3, 1H), 5.89 (s, 2H), 4.36 (q, J=6.9, 1H), 1.35 (t, J=7.2, 3H); LRMS calcd for C21H16Cl2N2O2 (M+H) 399, found 399.0. was cooled in an ice bath under nitrogen dissolved (approx 10 min) with stirring the solvent was removed in vacuo