methyl (6Z,9Z)-octadeca-6,9-dienoate

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The lipid thus obtained contains omega 9 type polyunsaturated fatty acid as a component of the lipid, such as fat. Although omega 9 type polyunsaturated fatty acid can be directly isolated, preferably it is isolated as an ester with a lower alcohol, for example, as methyl 8,11-eicosadienate, methyl 6,9-octadecadienoate, methyl ester of mead acid and the like. The esterification accelerates the separation of the target fatty acid from other lipid components, and from other fatty acids produced during the culturing, such as palmitic acid, oleic acid and linoleic acid (these fatty acids are also esterified simultaneously with the esterification of omega 9 type polyunsaturated fatty acid). For example, to obtain methyl ester of omega 9 type polyunsaturated fatty acid, the above-mentioned extract is treated with anhydrous methanol/HCl 5 to 10%, or BF3 /methanol 10 to 50% at room temperature for 1 to 24 hours. the separation of the target fatty acid from other lipid components

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The lipid thus obtained contains omega 9 type polyunsaturated fatty acid as a component of the lipid, such as fat. Although omega 9 type polyunsaturated fatty acid can be directly isolated, preferably it is isolated as an ester with a lower alcohol, for example, as methyl 8,11-eicosadienate, methyl 6,9-octadecadienoate, methyl ester of mead acid and the like. The esterification accelerates the separation of the target fatty acid from other lipid components, and from other fatty acids produced during the culturing, such as palmitic acid, oleic acid and linoleic acid (these fatty acids are also esterified simultaneously with the esterification of omega 9 type polyunsaturated fatty acid). For example, to obtain methyl ester of omega 9 type polyunsaturated fatty acid, the above-mentioned extract is treated with anhydrous methanol/HCl 5 to 10%, or BF3 /methanol 10 to 50% at room temperature for 1 to 24 hours. the separation of the target fatty acid from other lipid components