tert-butyl 2-aminopiperidine-1-carboxylate

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of the product from Example 219 (0.3 g) in tetrahydrofuran (2 ml) containing 4 amino-1-piperidinecarboxylic acid, 1,1-dimethylethyl ester (0. 161 g) and N-ethyldiisopropylamine (0.5 ml) was stirred for 16 hours before evaporating to dryness. The residue was taken into dichloromethane (30 ml) and trifluoroacetic acid (3 ml) added. The solution was stirred for 30 minutes then concentrated to give the title compound (310 mg). before evaporating to dryness added The solution was stirred for 30 minutes then concentrated

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of the product from Example 219 (0.3 g) in tetrahydrofuran (2 ml) containing 4 amino-1-piperidinecarboxylic acid, 1,1-dimethylethyl ester (0. 161 g) and N-ethyldiisopropylamine (0.5 ml) was stirred for 16 hours before evaporating to dryness. The residue was taken into dichloromethane (30 ml) and trifluoroacetic acid (3 ml) added. The solution was stirred for 30 minutes then concentrated to give the title compound (310 mg). before evaporating to dryness added The solution was stirred for 30 minutes then concentrated

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of the product from Example 219 (0.3 g) in tetrahydrofuran (2 ml) containing 4 amino-1-piperidinecarboxylic acid, 1,1-dimethylethyl ester (0. 161 g) and N-ethyldiisopropylamine (0.5 ml) was stirred for 16 hours before evaporating to dryness. The residue was taken into dichloromethane (30 ml) and trifluoroacetic acid (3 ml) added. The solution was stirred for 30 minutes then concentrated to give the title compound (310 mg). before evaporating to dryness added The solution was stirred for 30 minutes then concentrated

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: To a flask containing 0.6 g of a substrate N-Boc-3-piperidinone, 0.82 g of D-glucose, 4 mg of NAD+, 1.62 g of L-alanine, and 4.0 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNTPSPAG), obtained in Example 10, which expresses TPS, PALDH, and GDH was added so that total volume was 30 ml. The resultant product was stirred at 30° C. for 20 hours while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. The amount of N-Boc-aminopiperidine produced and the optical purity thereof were measured according to the method described in Example 19. In the result, the amount produced was 0.54 g. The absolute configuration of N-Boc-aminopiperidine was (S), and the optical purity was 99.4% e.e. (pNTPSPAG), obtained in Example 10, which was added so that total volume was 30 ml

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of the product from Example 219 (0.3 g) in tetrahydrofuran (2 ml) containing 4 amino-1-piperidinecarboxylic acid, 1,1-dimethylethyl ester (0. 161 g) and N-ethyldiisopropylamine (0.5 ml) was stirred for 16 hours before evaporating to dryness. The residue was taken into dichloromethane (30 ml) and trifluoroacetic acid (3 ml) added. The solution was stirred for 30 minutes then concentrated to give the title compound (310 mg). before evaporating to dryness added The solution was stirred for 30 minutes then concentrated