3-(3,5-difluorophenyl)-3-oxopropanenitrile

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: This compound was synthesized according to the procedure of Example 33 from 205 mg (1.13 mmol) of 3,5-difluorobenzoyl-acetonitrile and 300 mg (0.93 mmol) of 3-(4-chlorophenyl)-2(S)(3-cyanophenyl)-1(S)-methyl-propylamine hydrochloride. LC-MS: m/e=450 (M+1), 452 (M+3) (3.55 min). This compound was synthesized m/e=450 (M+1), 452 (M+3) (3.55 min)

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: This compound was synthesized according to the procedure of Example 33 from 205 mg (1.13 mmol) of 3,5-difluorobenzoyl-acetonitrile and 300 mg (0.93 mmol) of 3-(4-chlorophenyl)-2(S)(3-cyanophenyl)-1(S)-methyl-propylamine hydrochloride. LC-MS: m/e=450 (M+1), 452 (M+3) (3.55 min). This compound was synthesized m/e=450 (M+1), 452 (M+3) (3.55 min)

Conditions: temp:-20.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Diisopropylamine (10.6 mL, 76 mmol) was dissolved in dry THF (10 mL) and the solution cooled to −20° C. A solution of n-butyllithium (2.5N in hexanes, 30.5 mL, 76 mmol) was added slowly at such a rate as to keep the internal temperature below 0° C. After stirring for 5 min, the solution was cooled to −20° C. and added to a solution of acetonitrile (2.37 mL, 45.3 mmol) and ethyl 3,5-difluorobenzoate (6.23 g, 36.2 mmol) in dry THF (18 mL) at such a rate as to keep the temperature below −40° C. A further 10 mL of dry THF was used to transfer the remaining lithium diisopropylamide to the reaction. The reaction was allowed to warm to ambient over 2 h before being quenched with saturated aqueous ammonium chloride solution. The reaction was extracted with ethyl acetate (×2) and the extracts discarded. The aqueous was acidified with 2N HCl and extracted with ethyl acetate. The organic extract was washed with 2N HCl and brine before being dried over MgSO4, filtered and evaporated. Purification using chromatography (silica gel, gradient 5 to 100% ethyl acetate/isohexane) gave 3-(3,5-difluorophenyl)-3-oxopropanenitrile as a yellow solid (2.82 g). the internal temperature below 0° C the solution was cooled to −20° C. the temperature below −40° C to the reaction to warm to ambient over 2 h before being quenched with saturated aqueous ammonium chloride solution The reaction was extracted with ethyl acetate (×2) extracted with ethyl acetate The organic extract was washed with 2N HCl and brine before being dried over MgSO4 filtered evaporated Purification

Conditions: temp:-20.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Diisopropylamine (10.6 mL, 76 mmol) was dissolved in dry THF (10 mL) and the solution cooled to −20° C. A solution of n-butyllithium (2.5N in hexanes, 30.5 mL, 76 mmol) was added slowly at such a rate as to keep the internal temperature below 0° C. After stirring for 5 min, the solution was cooled to −20° C. and added to a solution of acetonitrile (2.37 mL, 45.3 mmol) and ethyl 3,5-difluorobenzoate (6.23 g, 36.2 mmol) in dry THF (18 mL) at such a rate as to keep the temperature below −40° C. A further 10 mL of dry THF was used to transfer the remaining lithium diisopropylamide to the reaction. The reaction was allowed to warm to ambient over 2 h before being quenched with saturated aqueous ammonium chloride solution. The reaction was extracted with ethyl acetate (×2) and the extracts discarded. The aqueous was acidified with 2N HCl and extracted with ethyl acetate. The organic extract was washed with 2N HCl and brine before being dried over MgSO4, filtered and evaporated. Purification using chromatography (silica gel, gradient 5 to 100% ethyl acetate/isohexane) gave 3-(3,5-difluorophenyl)-3-oxopropanenitrile as a yellow solid (2.82 g). the internal temperature below 0° C the solution was cooled to −20° C. the temperature below −40° C to the reaction to warm to ambient over 2 h before being quenched with saturated aqueous ammonium chloride solution The reaction was extracted with ethyl acetate (×2) extracted with ethyl acetate The organic extract was washed with 2N HCl and brine before being dried over MgSO4 filtered evaporated Purification

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: This compound was synthesized according to the procedure of Example 33 from 205 mg (1.13 mmol) of 3,5-difluorobenzoyl-acetonitrile and 300 mg (0.93 mmol) of 3-(4-chlorophenyl)-2(S)(3-cyanophenyl)-1(S)-methyl-propylamine hydrochloride. LC-MS: m/e=450 (M+1), 452 (M+3) (3.55 min). This compound was synthesized m/e=450 (M+1), 452 (M+3) (3.55 min)