ethyl 5-amino-1H-pyrrole-2-carboxylate

Conditions: temp:25.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A mixture of chiral-(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carboxylic acid (200 mg, 0.428 mmol), ethyl 5-amino-1H-pyrrole-2-carboxylate (180 mg, 1.17 mmol), 2-(7-azabenzotriazol-1-yl)-n,n,n′,n′-tetramethyluronium hexafluorophosphate (HATU, 200 mg, 0.526 mmol) and iPr2NEt (0.4 mL, 2.29 mmol) in CH2Cl2 (4 mL) and THF (4 mL) was stirred at 25° C. for 16 hours. The mixture was then diluted with CH2Cl2 and washed with water. The organic phase was separated, then concentrated under reduced pressure. The compound was purified by RP-HPLC (20%-95% of acetonitrile/water) to afford chiral 5-{[(2R,3S,4R,5S)-3-(3-chloro-2-fluoro-phenyl)-4-(4-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-1H-pyrrole-2-carboxylic acid ethyl ester (42 mg, 16.3%) as an off-white powder. HRMS (ES) m/z Calcd for C30H30Cl2F2N4O3+H [(M+H)+]: 603 736. found: 603.1736. washed with water The organic phase was separated concentrated under reduced pressure The compound was purified by RP-HPLC (20%-95% of acetonitrile/water)