(2S)-2-[2-[[(2S)-1-hydroxybutan-2-yl]amino]ethylamino]butan-1-ol

Conditions: temp:-80.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Antimicrobial drugs isoniazid, rifampicin, streptomycin, and ethambutol were purchased from Sigma-Aldrich. St. Louis, Mo. Stock solutions of isoniazid, streptomycin, and ethambutol were prepared in distilled and deionized water at 10 mg/mL, sterilized by filtration, and stored frozen at −80° C. Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol and stored at −80° C. Pyrazinamide was purchased as the drug reconstituting kit from Becton Dickinson, Cockeysville, Md., and a stock solution was prepared following instructions by the manufacturer. Stock solutions of SQ109 were prepared in methanol at 1 mg/mL and stored at 80° C. in distilled deionized water at 10 mg/mL, sterilized by filtration Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol stored at −80° C , and a stock solution was prepared stored at 80° C.

Conditions: temp:-80.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Antimicrobial drugs isoniazid, rifampicin, streptomycin, and ethambutol were purchased from Sigma-Aldrich. St. Louis, Mo. Stock solutions of isoniazid, streptomycin, and ethambutol were prepared in distilled and deionized water at 10 mg/mL, sterilized by filtration, and stored frozen at −80° C. Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol and stored at −80° C. Pyrazinamide was purchased as the drug reconstituting kit from Becton Dickinson, Cockeysville, Md., and a stock solution was prepared following instructions by the manufacturer. Stock solutions of SQ109 were prepared in methanol at 1 mg/mL and stored at 80° C. in distilled deionized water at 10 mg/mL, sterilized by filtration Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol stored at −80° C , and a stock solution was prepared stored at 80° C.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Ethambutol Dihydrochloride (ETB) is obtained from MP Biomedicals (Aurora, Ohio). ETB (7 grams) are dissolved in 100 mL of a saturated solution of sodium carbonate in 5M NaOH. The solution is stirred for 10 minutes and the free based ETB is extracted with three volumes of chloroform. The solution is dried over sodium sulfate. After filtration, the chloroform is removed by rotary evaporation. Ethambutol is represented in the structure below (a). is extracted with three volumes of chloroform The solution is dried over sodium sulfate After filtration the chloroform is removed by rotary evaporation

Conditions: temp:-80.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Antimicrobial drugs isoniazid, rifampicin, streptomycin, and ethambutol were purchased from Sigma-Aldrich. St. Louis, Mo. Stock solutions of isoniazid, streptomycin, and ethambutol were prepared in distilled and deionized water at 10 mg/mL, sterilized by filtration, and stored frozen at −80° C. Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol and stored at −80° C. Pyrazinamide was purchased as the drug reconstituting kit from Becton Dickinson, Cockeysville, Md., and a stock solution was prepared following instructions by the manufacturer. Stock solutions of SQ109 were prepared in methanol at 1 mg/mL and stored at 80° C. in distilled deionized water at 10 mg/mL, sterilized by filtration Stock solutions of rifampicin, at 1 or 10 mg/mL, were prepared in methanol stored at −80° C , and a stock solution was prepared stored at 80° C.