PENTAFLUOROPHENYL ACRYLATE

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: (2,3,4,5,6-Pentafluorophenyl) prop-2-enoate (0.030 mL, 0.19 mmol) was added dropwise to a solution of 4-methoxy-6-(4-methylpiperazin-1-yl)-N′(4-pyrazolo[1,5-a]-pyridin-3-ylpyrimidin-2-yl)benzene-1,3-diamine (Intermediate 69, 67 mg, 0.16 mmol) in DMF (0.6 mL) at r.t. under N2. The resulting solution was stirred at r.t. for 1.5 h and then diluted with CH2Cl2 (9 mL). This solution was added to 1.5 g flash silica which was wet with CH2Cl2 in a dry-loaded cartridge, and the crude product was eluted from the silica using CH2Cl2. Further purification by FCC, eluting with 2-7% of 2N methanolic ammonia in CH2Cl2 provided material which was further purified by FCC, eluting with 5-20% CH3OH in CH2Cl2. Appropriate fractions were concentrated in vacuo and trituration provided a crystalline solid that was washed with THF (0.1 mL) to give the title compound (23 mg, 28%) as a beige crystalline solid; 1H NMR: 2.33-2.43 (3H, m), 2.62-2.81 (4H, m), 2.94 (4H, s), 3.85 (3H, s), 5.72 (1H, d), 6.18 (1H, m), 6.55-6.69 (1H, m), 6.89 (1H, s), 7.05 (1H, m), 7.26 (1H, d), 7.35-7.43 (1H, m), 8.15 (1H, s), 8.34 (1H, d), 8.44 (1H, d), 8.49 (1H, s), 8.79 (1H, d), 8.80 (1H, s), 9.03 (1H, s); m/z: ES+ MH+ 485. This solution was added to 1.5 g flash silica which the crude product was eluted from the silica Further purification by FCC eluting with 2-7% of 2N methanolic ammonia in CH2Cl2 provided material which was further purified by FCC eluting with 5-20% CH3OH in CH2Cl2 Appropriate fractions were concentrated in vacuo and trituration provided a crystalline solid that was washed with THF (0.1 mL)