2-tetradecylhexadecanoic Acid

Conditions: temp:55.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 144 mg of 2-(tetradecyl)hexadecanoic acid was dissolved in 25 ml of methanol. After the addition of a few drops of concentrated sulfuric acid, the solution was stirred at 55° C. for one day, neutralized with 10% sodium hydroxide and extracted with dichloromethane. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50) to obtain methyl 2-(tetradecyl)hexadecanoate. extracted with dichloromethane The organic layer was washed with water dried over anhydrous sodium sulfate concentrated in vacuo The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50)

Conditions: temp:55.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 144 mg of 2-(tetradecyl)hexadecanoic acid was dissolved in 25 ml of methanol. After the addition of a few drops of concentrated sulfuric acid, the solution was stirred at 55° C. for one day, neutralized with 10% sodium hydroxide and extracted with dichloromethane. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50) to obtain methyl 2-(tetradecyl)hexadecanoate. extracted with dichloromethane The organic layer was washed with water dried over anhydrous sodium sulfate concentrated in vacuo The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50)

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 1.0 g of 2-tetradecylhexadecanoic acid and 0.79 g of thionyl chloride in 5 ml of benzene was refluxed for 7 hours. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue and then benzene was removed by distillation. This procedure was repeated 3 times to remove thionyl chloride completely. The residue was recrystallized from anhydrous hexane to obtain 0.75 g of 2-tetradecylhexadecanoyl chloride having a melting point of 51°-53° C. To the solution of 0.85 g of 1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester in 35 ml of anhydrous pyridine, was added 4.9 g of 2-tetradecylhexadecanoyl chloride dissolved in 35 ml of anhydrous tetrahydrofuran at 17°-18° C. After 45 minutes, 20 ml of water was added and the solution was stirred for 35 minutes at room temperature. The solution was adjusted to pH 3 with 1 M hydrochloric acid and extracted with chloroform. The chloroform solution was washed with saturated brine, and dried. After removal of solvent, the residue was subjected to silica gel column chromatography. Eluate with a mixture of chloroform and methanol (20:1) was concentrated and the residue was recrystallized from methanol to obtain 0.75 g of 6-O-(2-tetradecylhexadecanoyl)-1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester having a melting point of 173°-174° C. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue benzene was removed by distillation to remove thionyl chloride completely The residue was recrystallized from anhydrous hexane

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 1.0 g of 2-tetradecylhexadecanoic acid and 0.79 g of thionyl chloride in 5 ml of benzene was refluxed for 7 hours. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue and then benzene was removed by distillation. This procedure was repeated 3 times to remove thionyl chloride completely. The residue was recrystallized from anhydrous hexane to obtain 0.75 g of 2-tetradecylhexadecanoyl chloride having a melting point of 51°-53° C. To the solution of 0.85 g of 1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester in 35 ml of anhydrous pyridine, was added 4.9 g of 2-tetradecylhexadecanoyl chloride dissolved in 35 ml of anhydrous tetrahydrofuran at 17°-18° C. After 45 minutes, 20 ml of water was added and the solution was stirred for 35 minutes at room temperature. The solution was adjusted to pH 3 with 1 M hydrochloric acid and extracted with chloroform. The chloroform solution was washed with saturated brine, and dried. After removal of solvent, the residue was subjected to silica gel column chromatography. Eluate with a mixture of chloroform and methanol (20:1) was concentrated and the residue was recrystallized from methanol to obtain 0.75 g of 6-O-(2-tetradecylhexadecanoyl)-1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester having a melting point of 173°-174° C. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue benzene was removed by distillation to remove thionyl chloride completely The residue was recrystallized from anhydrous hexane

Conditions: temp:55.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 144 mg of 2-(tetradecyl)hexadecanoic acid was dissolved in 25 ml of methanol. After the addition of a few drops of concentrated sulfuric acid, the solution was stirred at 55° C. for one day, neutralized with 10% sodium hydroxide and extracted with dichloromethane. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50) to obtain methyl 2-(tetradecyl)hexadecanoate. extracted with dichloromethane The organic layer was washed with water dried over anhydrous sodium sulfate concentrated in vacuo The residue was purified by silica gel chromatography (ethyl acetate:hexane=1:50)

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 1.0 g of 2-tetradecylhexadecanoic acid and 0.79 g of thionyl chloride in 5 ml of benzene was refluxed for 7 hours. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue and then benzene was removed by distillation. This procedure was repeated 3 times to remove thionyl chloride completely. The residue was recrystallized from anhydrous hexane to obtain 0.75 g of 2-tetradecylhexadecanoyl chloride having a melting point of 51°-53° C. To the solution of 0.85 g of 1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester in 35 ml of anhydrous pyridine, was added 4.9 g of 2-tetradecylhexadecanoyl chloride dissolved in 35 ml of anhydrous tetrahydrofuran at 17°-18° C. After 45 minutes, 20 ml of water was added and the solution was stirred for 35 minutes at room temperature. The solution was adjusted to pH 3 with 1 M hydrochloric acid and extracted with chloroform. The chloroform solution was washed with saturated brine, and dried. After removal of solvent, the residue was subjected to silica gel column chromatography. Eluate with a mixture of chloroform and methanol (20:1) was concentrated and the residue was recrystallized from methanol to obtain 0.75 g of 6-O-(2-tetradecylhexadecanoyl)-1-α-O-benzyl-N-acetylmuramyl-L-alanyl-D-isoglutamine benzyl ester having a melting point of 173°-174° C. After removal of benzene and thionyl chloride, anhydrous benzene was added to the residue benzene was removed by distillation to remove thionyl chloride completely The residue was recrystallized from anhydrous hexane