(8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-1,2,4,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The optimum pH for the production of cholestenone when epicholesterol is used as the substrate, is 8-12. The optimum pH when epicholesterol is produced from 5-cholesten-3-one, is 4-5. Determined at 37° C. using various pH buffer, 0.1M acetate/hydrochloride buffer (pH 2-4), 0.1M phosphate/citrate buffer (pH 4-7), 0.1M Tris-HCl buffer (pH 7-9), and 0.1M glycine/sodium hydroxide buffer (pH 9-12), each containing 1 mM dithiothreitol!.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: The optimum pH for the production of cholestenone when epicholesterol is used as the substrate, is 8-12. The optimum pH when epicholesterol is produced from 5-cholesten-3-one, is 4-5. [Determined at 37° C. using various pH buffer, 0.1M acetate/hydrochloride buffer (pH 2-4), 0.1 M phosphate/citrate buffer (pH 4-7), 0.1 M Tris-HCl buffer (pH 7-9), and 0.1 M glycine/sodium hydroxide buffer (pH 9-12), each containing 1 mM dithiothreitol].