(8S,9S,10R,11S,13S,14S,16S,17R)-11-chloro-17-hydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 11β-chloro-17,21-dihydroxy-16β-methylpregna-1,4-diene-3,20-dione (214 mg.) in dioxan (7 ml.) was treated with trimethyl orthoisobutyrate (0.22 ml.) and toluene p-sulphonic acid (23 mg.) at room temperature. After 45 minutes a little sodium bicarbonate was added and the mixture was then poured into a dilute solution of sodium bicarbonate. The oily product was extracted into ethyl acetate and washed with water. Evaporation of the organic solent gave an oil which was dissolved in acetone (10 ml.) and treated with 0.08 N-sulphuric acid (2.4 ml.). After keeping at room temperature for 30 minutes, the solution was diluted with water and the precipitated oil was extracted with ethyl acetate and washed with water. Evaporation of the organic solvent afforded the crude product which, on crystallization from ether-petroleum ether, gave the title compound, m.p. 163°-164° (K), [α]D + 108.5 (c 0.6 chloroform) λmax 240 nm (ε 15,460) (Found: C, 67.7; H, 7.7; Cl, 7.4. C26H35Cl O5 requires C, 67.45; H, 7.6; Cl, 7.65%). The oily product was extracted into ethyl acetate washed with water Evaporation of the organic solent gave an oil which the precipitated oil was extracted with ethyl acetate washed with water Evaporation of the organic solvent afforded the crude product which on crystallization from ether-petroleum ether

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 21-acetoxy-11β-chloro-17-hydroxy-16β-methylpregna-1,4-diene-3,20-dione (100 mg.) in methanol (7 ml.) was treated with 60% aqueous perchloric acid (0.2 ml.) at room temperature. After 24 hours sodium acetate (100 mg.) was added and about half of the solvent was evaporated in vacuo. Purification of the remaining solution by preparative thin layer chromatography and crystallisation from methanol afforded 11β-chloro-17,21-dihyroxy-16β-methylpregna-1,4-diene-3,20-dione as needles, m.p. 202°-204° decomp (Kofler). was evaporated in vacuo Purification of the remaining solution by preparative thin layer chromatography and crystallisation from methanol