5-[(2-methylpropan-2-yl)oxycarbonyl]-5-azaspiro[2.4]heptane-6-carboxylic acid

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM

Conditions: temp:25.01; pressure:0.00; stir:

Procedure: 150 uL of MeCN/H2O (1:1) mixed solvent was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 150 uL of internal standard solution was dispensed separately into each plate using the 96-tip head of the Tecan Evo-200. 10 uL aliquot taken from reaction vials, and added to pre-filled wells in the transfer plate. 380 uL MeCN solvent were added to the transfer plate 20 uL taken from transfer plate to filter plate 1 for LCMS analysis 180 uL MeCN added to filter plate 1 for LCMS analysis 10 uL taken from transfer plate to filter plate 2 for TOF-DESI analysis 190 uL MeCN added to filter plate 2 for TOF-DESI analysis the 96-well filter plates were centrifuged for 2.5 min at 3000 RPM