magnesium;diacetate

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: An aqueous magnesium acetate solution was prepared by adding 1.07 g of magnesium acetate tetrahydrate as a Group 2 or 3 element compound to 30 g of ion-exchanged water and then stirring this solution with a homomixer T. K. ROBOMIX (manufactured by PRIMIX Corporation). Then, 0.44 g of 3-aminopropyltriethoxysilane (KBE-903 manufactured by Shin-Etsu Chemical Co., Ltd.) as a silane coupling agent was gradually added to the aqueous solution. This mixture was subsequently stirred for 5 hours to induce hydrolysis and a condensation reaction of the silane coupling agent, thereby producing a suspension containing a composite compound which contained magnesium and silicon. This mixture was subsequently stirred for 5 hours hydrolysis producing a suspension containing a composite compound which

Conditions: temp:55.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 2.17 parts of N-acetoacetyl-L-aspartic acid, 1.02 parts by volume of acetic anhydride, and 0.014 parts of magnesium acetate were mixed with 40 parts by volume of ethyl acetate and heated at 55±2° C. under nitrogen for 24 hours. 30 parts by volume of methanol was added and the mixture stirred at ambient temperatures for 5 hours. HPLC analysis of the resulting solution showed only 0.5% unreacted N-acetoacetyl-L-aspartic acid.

Conditions: temp:55.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: 21.72 parts of N-acetoacetyl-L-aspartic acid, 0.14 parts of magnesium acetate, and 9.5 parts by volume of acetic anhydride were mixed with 200 parts by volume of ethyl acetate and heated at 55±2° C. under argon for 24 hours.

Conditions: temp:50.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A magnesium bicarbonate solution was prepared from hydromagnesite (15.0 g) and magnesium acetate (17.0 g) in water (circa 1000 mL) by bubbling carbon dioxide through the aqueous suspension overnight. [Minor residual undissolved solids derived from the hydromagnesite used were not removed by filtration.] The resulting solution was heated to 50° C. with magnetic stirring and aqueous KOH was added while continuing the magnetic stirring. Five different experiments were run, and in each case, the amount of KOH was varied and the final pH was measured and is shown in Table II. The precipitate was filtered and dried in an oven at about 110° C., resulting in a solid material which was examined by TGA to determine the hydromagnesite/magnesium hydroxide ratio, and by SEM to determine the morphology and size of product. The materials were also evaluated for their ease in filtration. These results are summarized in Table II. The X-ray powder pattern of brucite was not observed in the X-ray powder patterns (XRD) of the samples with pH<10.6, indicating that the magnesium hydroxide has a very small crystalline size, i.e., <50 Å. The XRD of the sample at pH=10.61 showed broad brucite peaks. SEM analysis of these samples showed neither spheres nor rosettes, due to the agitation during the base addition. by bubbling carbon dioxide through the aqueous suspension overnight used were not removed by filtration aqueous KOH was added The precipitate was filtered dried in an oven at about 110° C. resulting in a solid material which The materials were also evaluated for their ease in filtration due to the agitation during the base addition

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: ADP substrate (>99.999% pure with respect to ATP), magnesium acetate and extractant reagent (0.15% cationic detergent and 0.25% tertiary diamine per final volume) is supplied from source (10) in sufficient quantity to provide 0.1 mM ADP and 10 mM magnesium ions in the reaction vessel, the magnet deenergised and stirrer operated and extraction/ADP conversion allowed to proceed for a set period between of 1 or 5 minutes before the stirrer is stopped, the magnet energised and the liquid removed to the luminometer flow cell (11) using the peristaltic tubing (3) and reversible pumps (4) and (12) while simultaneously mixing it with an effective volume of luciferase/luciferin reagent of depleted adenylate kinase content from source (13). Using an adenylate kinase modified Celsis LDR reagent of otherwise standard amounts of active ingredients this volume is about one half of the volume of the sample. Light emitted in the flow cell is related to the amount of E. coli captured by reference to standard curves obtained by performance of the assay with known numbers of cells.

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution consisting of 52.8 g of L-ascorbic acid, 965 ml of water and 37 ml of pyridine was cooled to 0°-5° C., and 71 g of phosphoryl chloride was added dropwise with stirring over a period of about 2 hours while maintaining the pH at 12.7±0.2 with a 50% aqueous potassium hydroxide solution. After completion of the dropwise addition, the resulting mixture was stirred at the same temperature for about 30 minutes. After completion of the reaction, the pyridine was distilled off under reduced pressure at about 40° C., and the residue was adjusted to a pH of about 1 with concentrated hydrochloric acid and then diluted with water to adjust the L-ascorbic acid-2-phosphate concentration to about 2%, whereby a starting solution for adsorption was prepared. The starting solution for adsorption was cooled to 0°-5° C. and passed through a column of activated carbon cooled to 0°-5° C. [a column with a diameter of 10 cm and a length of 30 cm which had been packed with activated carbon (Ryujo Shirasagi, mfd. by Takeda Chemical Industries, Ltd.) by a wet process so that the length of packed portion of the column might be 20 cm] at a flow rate SV=1 to be subjected to adsorption. Then, a 0.5% aqueous hydrochloric acid solution was passed through the column under the same conditions as above until the electric conductivity of the solution became 20 ms or less. Subsequently, a 2% aqueous magnesium acetate solution was passed through the column at a flow rate SV=1 at room temperature to elute a desired component (magnesium L-ascorbic acid-2-phosphate). The eluate thus obtained was monitored by means of absorbance and a fraction containing 0.1% or more of the desired component was separated therefrom as an available fraction. The available fraction was concentrated to adjust the concentration of the desired component to about 10%, after which acetone was added in a volume of twice that of the residue with stirring to precipitate magnesium L-ascorbic acid-2-phosphate. The crystals precipitated were collected by filtration and then dried by air blowing at room temperature to obtain 65 g of magnesium L-ascorbic acid-2-phosphate. The magnesium L-ascorbic acid-2-phosphate obtained had a purity of as good as 98% (in terms of dehydrate) and an absorbance of as good as 0.01 or less (360 nm, C=1%). was cooled to 0°-5° C. After completion of the dropwise addition the resulting mixture was stirred at the same temperature for about 30 minutes After completion of the reaction the pyridine was distilled off under reduced pressure at about 40° C. diluted with water the L-ascorbic acid-2-phosphate concentration to about 2%, whereby a starting solution for adsorption was prepared The starting solution for adsorption was cooled to 0°-5° C. cooled to 0°-5° C. [a column with a diameter of 10 cm became 20 ms or less was passed through the column at a flow rate SV=1 at room temperature to elute a desired component (magnesium L-ascorbic acid-2-phosphate) The eluate thus obtained a fraction containing 0.1% or more of the desired component was separated The available fraction was concentrated after which acetone was added in a volume of twice that of the residue with stirring to precipitate magnesium L-ascorbic acid-2-phosphate The crystals precipitated were collected by filtration dried by air blowing at room temperature

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: An aqueous magnesium acetate solution was prepared by adding 1.07 g of magnesium acetate tetrahydrate as a Group 2 or 3 element compound to 30 g of ion-exchanged water and then stirring this solution with a homomixer T. K. ROBOMIX (manufactured by PRIMIX Corporation). Then, 0.44 g of 3-aminopropyltriethoxysilane (KBE-903 manufactured by Shin-Etsu Chemical Co., Ltd.) as a silane coupling agent was gradually added to the aqueous solution. This mixture was subsequently stirred for 5 hours to induce hydrolysis and a condensation reaction of the silane coupling agent, thereby producing a suspension containing a composite compound which contained magnesium and silicon. This mixture was subsequently stirred for 5 hours hydrolysis producing a suspension containing a composite compound which

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: An aqueous magnesium acetate solution was prepared by adding 1.07 g of magnesium acetate tetrahydrate as a Group 2 or 3 element compound to 30 g of ion-exchanged water and then stirring this solution with a homomixer T. K. ROBOMIX (manufactured by PRIMIX Corporation). Then, 0.44 g of 3-aminopropyltriethoxysilane (KBE-903 manufactured by Shin-Etsu Chemical Co., Ltd.) as a silane coupling agent was gradually added to the aqueous solution. This mixture was subsequently stirred for 5 hours to induce hydrolysis and a condensation reaction of the silane coupling agent, thereby producing a suspension containing a composite compound which contained magnesium and silicon. This mixture was subsequently stirred for 5 hours hydrolysis producing a suspension containing a composite compound which

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Into a 300 ml. flask equipped as in EXAMPLE I and maintained under a nitrogen atmosphere were added 21.5 grams magnesium acetate tetrahydrate and 80 ml. acetic anhydride and the mixture was heated at the reflux temperature for 1 hour. After cooling, a clear liquid was drawn off and the remaining solid was dried at 35° C. under a vacuum of 0.3 mm mercury for several hours to obtain anhydrous magnesium acetate. flask equipped as in EXAMPLE I maintained under a nitrogen atmosphere After cooling the remaining solid was dried at 35° C. under a vacuum of 0.3 mm mercury for several hours

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Into a 300 ml. flask equipped with mechanical stirrer and a condenser, and maintained under a nitrogen atmosphere, were added 21.5 grams magnesium acetate tetrahydrate and 100 ml. acetic anhydride and the mixture was heated at the reflux temperature for 3 hours. After the mixture had cooled, the liquid was drawn off and the solid dried at 40° C. under a vacuum of 0.2 mm mercury for several hours to obtain anhydrous magnesium acetate. flask equipped with mechanical stirrer and a condenser maintained under a nitrogen atmosphere After the mixture had cooled the solid dried at 40° C. under a vacuum of 0.2 mm mercury for several hours

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Into a 300 ml. flask equipped as in EXAMPLE I and maintained under a nitrogen atmosphere were added 21.5 grams magnesium acetate tetrahydrate and 80 ml. acetic anhydride and the mixture was heated at the reflux temperature for 1 hour. After cooling, a clear liquid was drawn off and the remaining solid was dried at 35° C. under a vacuum of 0.3 mm mercury for several hours to obtain anhydrous magnesium acetate. flask equipped as in EXAMPLE I maintained under a nitrogen atmosphere After cooling the remaining solid was dried at 35° C. under a vacuum of 0.3 mm mercury for several hours

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Into a 300 ml. flask equipped with mechanical stirrer and a condenser, and maintained under a nitrogen atmosphere, were added 21.5 grams magnesium acetate tetrahydrate and 100 ml. acetic anhydride and the mixture was heated at the reflux temperature for 3 hours. After the mixture had cooled, the liquid was drawn off and the solid dried at 40° C. under a vacuum of 0.2 mm mercury for several hours to obtain anhydrous magnesium acetate. flask equipped with mechanical stirrer and a condenser maintained under a nitrogen atmosphere After the mixture had cooled the solid dried at 40° C. under a vacuum of 0.2 mm mercury for several hours