(2S)-2-amino-N-benzyl-3-(1H-indol-3-yl)propanamide

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112°-114° C.; 1H NMR (300 MHz, CDCl3) δ8.40-7.00 (m, 11H, Ar), 4.44 (d, J=6 Hz, 2H, CH2 --N), 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, 1H, H-β), 2.98 (dd, J=9, 14 Hz, 1H, H-β); mass spectrum (EI), m/e 293 (M+), 277 (M+ -NH2), 130 (M+ --H2N--CH--CONH--Bn); [α]25D +42.1° (c=1.02, MeOH).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112°-114° C.; 1H NMR (300 MHz, CDCl3) δ8.40-7.00 (m, 11H, Ar), 4.44 (d, J=6 Hz, 2H, CH2 --N), 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, 1H, H-β), 2.98 (dd, J=9, 14 Hz, 1H, H-β); mass spectrum (EI), m/e 293 (M+), 277 (M+ -NH2), 130 (M+ --H2N--CH--CONH--Bn); [α]25D +42.1° (c=1.02, MeOH).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112°-114° C.; 1H NMR (300 MHz, CDCl3) δ8.40-7.00 (m, 11H, Ar), 4.44 (d, J=6 Hz, 2H, CH2 --N), 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, 1H, H-β), 2.98 (dd, J=9, 14 Hz, 1H, H-β); mass spectrum (EI), m/e 293 (M+), 277 (M+ -NH2), 130 (M+ -H2N--CH--CONH-Bn); [α]D25 +42.1° (c=1.02, MeOH).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112°-114° C.; 1H NMR (300 MHz, CDCl3) δ8.40-7.00 (m, 11H, Ar), 4.44 (d, J=6 Hz, 2H, CH2 --N), 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, 1H, H-β), 2.98 (dd, J=9, 14 Hz, 1H, H-β); mass spectrum (EI), m/e 293 (M+), 277 (M+ -NH2), 130 (M+ -H2N--CH--CONH-Bn); [α]D25 +42.1° (c=1.02, MeOH).

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: compound PSV11R. Same procedure as above with Trp-NHCH2Ph (82.43 mg, 0.281 mmol), N-Boc-Tyr(Bn)-Gly-OH (109.26 mg, 0.255 mmol), EDC (53.87 mg, 0.281 mmol) and HOBt (37.97 mg, 0.281 mmol) in CH2Cl2/DMF (2 mL/0.8 mL). The crude residue was triturated with CH2Cl2/pentane to afford a white solid (113.76, 63%). mp 183° C. 1H NMR (300 MHz, CDCl3) δ 1.38 (s, 9H, (CH3)3), 2.81 (m, 2H, CH2 Tyr), 3.26 (m, 2H), 3.72 (m, 2H, CH2 Gly), 4.30 (m, 3H, CHα and CH2Bn), 4.80 (m, 1H, CHα), 5.0 (broad s, 3H, NHBoc and CH2O), 6.72 (m, 1H, NH Gly), 6.90-7.40 (m, 20H, aromatic H and NH), 7.63 (d, 1H, J=7 Hz), 8.35 (s, 1H, NH indole). 13C NMR (75 MHz, CDCl3) δ 28.3 (CH2 Trp and (CH3)3), 37.5 (CH2 Tyr), 43.1, 43.5 (CH2 (Bn), CH2 Gly), 54.0, 55.8 (CHα Tyr, Trp), 70.0 (CH2 (Bn)), 80.4 (C(CH3)3), 110.2, 111.3, 114.9, 118.7, 119.5, 122.0, 123.4, 127.2, 127.4, 127.5, 127.7, 128.0, 128.5, 128.6, 128.7, 130.3, 136.1, 137.0, 137.9 (20 aromatic C), 155.7, 157.7 (Car-O, CO carbamate), 168.9, 171.4, 172.5 (3 CO amide). Anal. Calcd. for C41H45N5O6, 1H2O: C, 68.22; H, 6.56; N, 9.71. Found: C, 68.50; H, 6.48; N, 9.98. The crude residue was triturated with CH2Cl2/pentane to afford a white solid (113.76, 63%)

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: compound A416. Same procedure as above with Trp-NHCH2Ph (63.03 mg, 0.215 mmol), N-Boc-Tyr(Bn)-Asn-OH (105.0 mg, 0.216 mmol), EDC (44 mg, 0.226 mmol) and HOBt (31 mg, 0.226 mmol) in CH2Cl2/DMF (1.5 mL/1.5 mL). After treatment, the crude residue was triturated with CH2Cl2/pentane to afford a white solid (78 mg, 48%). 1H NMR (300 MHz, DMSO-d6) δ 1H NMR (300 MHz, DMSO-d6) δ 1.29 (s, 9H, (CH3)3), 2.30-3.26 (m, 6H, CH2 Tyr, CH2 Trp, CH2 Asn), 4.08 (m, 1H, CHα), 4.24 (s, 2H, NCH2Ph), 4.49 (m, 1H, CHα), 4.56 (m, 1H, CHα), 5.03 (broad s, 2H, OCH2Ph), 6.88-7.56 (m, 17H), 8.17 (d, J=8 Hz, 1H, NH), 8.23 (d, J=8 Hz, 1H, NH), 8.51 (t, J=6 Hz, 1H, NH), 10.84 (s, 1H, NH). 13C NMR (75 MHz, CD3OD) δ 28.5 (CH2 Trp), 28.6 ((CH3)3), 37.3, 38.1 (CH2 Tyr, CH2 Asn), 44.1 (NCH2Ph), 51.7, 55.9, 57.6 (CHα Tyr, Asn, Trp), 71.0 (OCH2Ph), 80.9 (C Boc), 110.9 (C), 112.4, 115.9, 116.0, 119.4, 119.9, 122.5, 124.8, 128.0, 128.5, 128.6, 128.8, 129.4, 129.5, 130.6 (C), 131.4 (CH), 138.0, 138.8, 139.5 (aromatic C), 157.9, 159.1 (aromatic C and CO Boc), 172.7, 173.6, 174.4, 174.9 (CO amide). After treatment, the crude residue was triturated with CH2Cl2/pentane

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: compound SP307P. Same procedure as above with Trp-NHCH2Ph (120.7 mg, 0.411 mmol), N-Boc-Tyr(Bn)-Lys(Boc)-OH (246.9 mg, 0.411 mmol), EDC (87.1 mg, 0.45 mmol) and HOBt (62.1 mg, 0.46 mmol) in CH2Cl2/DMF (1.8 mL, 1/1). The crude residue was triturated with MeOH/pentane to afford a white solid (243 mg, 67%). 1H NMR (300 MHz, DMSO-d6) δ 1.2-1.61 (m, 6H, 3 CH2 Lys), 1.29 (s, 9H, (CH3)3), 1.36 (s, 9H, (CH3)3), 2.64 (m, 1H, CH2 Tyr), 2.86 (m, 3H, CH2 Lys, CH2 Tyr), 3 (dd, J=14.1 Hz, J=7 Hz, 1H, CH2 Trp), 3.14 (dd, J=14.1 Hz, J=6 Hz, 1H, CH2 Trp), 4.11 (m, 1H, CHα), 4.26 (m, 3H, CH2 (Bn Trp), CHα), 4.59 (m, 1H, CHα), 5.02 (broad s, 2H, CH2 (Bn)), 6.72 (m, 1H), 6.85-7.4 (m, 19H), 7.59 (d, J=7.6 Hz, 1H), 7.89 (d, J=7.4 Hz, 1H), 8.1 (d, J=7.4 Hz, 1H), 8.38 (m, 1H), 10.8 (s, 1H). 13C NMR (75 MHz, DMSO-d6) δ 22.4, 29.2, 32 (3 CH2 Lys), 27.8 (CH2 Trp), 28.1, 28.2 (2 (CH3)3), 36.3 (CH2 Tyr), 39.8 (CH2 Lys), 42 (CH2 (Bn) Trp), 52.4, 53.5, 55.8 (CHα Tyr, Lys, Trp), 69.1 (CH2 (Bn)), 77.3, 78 (2 C(CH3)3), 109.7, 111.2, 114.3, 118.2, 118.4, 120.8, 123.5, 126.5, 126.9, 127.3, 127.5, 127.7, 128, 128.3, 130.1, 130.2, 136, 137.2, 139 (25 aromatic C), 155.2, 155.5, 156.8 (Car-O, 2 CO carbamate), 171, 171.3, 171.6 (3 CO amide). Anal. Calcd. for C50H62N6O, 2H2O: C, 65.91; H, 7.30; N, 9.22. Found: C, 65.59; H, 7.06; N, 9.09. The crude residue was triturated with MeOH/pentane

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: Same procedure as above with Z-Trp-NHCH2Ph (616 mg, 1.44 mmol), 10% Pd/C (153 mg) in MeOH/DMF (14.5 mL, 1/1). After purification by flash chromatography on silica gel (3-12% MeOH/CH2Cl2), Trp-NHCH2Ph was isolated as a pale yellow oil (351 mg, 83%). 1H NMR (300 MHz, CDCl3) δ 2.98 (dd, J=14.4 Hz, J=8.7 Hz, 1H, CH2), 3.44 (dd, J=14.4 Hz, J=4.1 Hz, 1H, CH2), 3.80 (dd, J=8.7 Hz, J=4.2 Hz, 1H, CHα), 4.46 (m, 2H, CH2 (Bn)), 7.06 (d, J=2.1 Hz, 1 aromatic H), 7.13-7.36 (m, 7 aromatic H), 7.41 (d, J=8 Hz, 1 aromatic H), 7.61 (m, 1H, NH amide), 7.72 (d, J=7.8 Hz, 1 aromatic H), 8.3 (broad s, 1H, NH indole). 13C NMR (75 MHz, MeOD) δ 30.5 (CH2 Trp), 42.7 (CH2 Ph), 55.4 (CHα), 109.3, 111, 118.1, 118.5, 121.2, 123.5, 126.8, 127, 127.1, 127.4, 128, 128.1, 136.8, 138 (14 aromatic C), 174.6 (CO amide). After purification by flash chromatography on silica gel (3-12% MeOH/CH2Cl2)

Conditions: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

Procedure: A solution of 2.20 g (5.15 mmol) Cbz-Trp-NHBn in 60 mL of MeOH was subjected to hydrogenolysis over Pd/C to give 1.43 g (4.88 mmol, 95%) of Trp-NHBn. Characteristic analytical data are as follows: mp 112°-114° C.; 1H NMR (300 MHz, CDCl3) δ8.40-7.00 (m, 11H, Ar), 4.44 (d, J=6 Hz, 2H, CH2 --N), 3.77 (dd, J=4 Hz, 9H, H-α), 3.42 (dd, J=4, 14 Hz, 1H, H-β), 2.98 (dd, J=9, 14 Hz, 1H, H-β); mass spectrum (EI), m/e 293 (M+), 277 (M+ -NH2), 130 (M+ --H2N--CH--CONH--Bn); [α]25D +42.1° (c=1.02, MeOH).